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Published on: June 11, 2018
Capillary blood microsampling to determine serum biopharmaceutical concentration: Mitra® microsampler vs dried blood
Karien Bloem1,2, Tiny Schaap2, Ronald Boshuizen2
1Department of Immunopathology, Sanquin Research & Landsteiner Laboratory Academic Medical Centre, 1006 CX Amsterdam, The Netherlands.
Aim:
For assessment of concentrations of biopharmaceuticals, for example, therapeutic drug monitoring, dried blood sampling of capillary blood is a convenient alternative to traditional venepuncture sampling. We investigated an alternative to dried blood spot collection on filter paper: sampling capillary blood using the Mitra® microsampler.
Materials And Methods:
Therapeutic monoclonal antibodies were spiked in whole blood, sampled using filter paper and Mitra microsampler and concentrations measured using specific ELISAs.
Results:
Good recoveries of adalimumab, infliximab, ustekinumab, vedolizumab, tocilizumab, natalizumab and rituximab were found up to 1 month of storage at room temperature, averaging 95.2% for the Mitra microsampler and 92.9% for Whatman® paper. Both hemoglobin and potassium yield satisfactory estimates for the volume of the cellular fraction of blood samples in combination with the Mitra microsampler.
Conclusion:
We established practical protocols for the estimation of serum/plasma concentrations of therapeutic antibodies via capillary blood microsampling.
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