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Updated: Feb 9, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
Novel and rapid LC-MS/MS method for quantitative analysis of methylphenidate in dried blood spots
Adarsh Gandhi1, Christopher Beekman2, Robert Parker1
1Office of Clinical Pharmacology, Center for Drugs Evaluation & Research, US Food & Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA.
Aim:
Development and validation of a novel, sensitive, specific and rapid dried blood spots (DBS)-LC-MS/MS method for methylphenidate (MPH), an attention-deficit hyperactivity disorder drug. Methodology & results: Protein precipitation with acetonitrile was used to extract MPH from the DBS cards. Chromatographic separation was achieved on a Zorbax C18 column using an isocratic mobile phase composed of acetonitrile and 5 mM ammonium formate buffer (20:80, v/v) at a flow rate of 0.5 ml/min. MPH was quantified over a linear range of 200-25,000 pg/ml.
Conclusion:
The clinical DBS-LC-MS/MS method was successfully validated as per the US FDA's Bioanalytical Method Validation Guidance to support an ongoing pediatric pharmacokinetic study.
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