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Updated: Feb 9, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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How to perform aggregometry and lumi-aggregometry in mouse platelets.

Craig E Hughes1

  • 1a Institute for Cardiovascular and Metabolic Research, School of Biological Sciences , University of Reading , Reading , UK.

Platelets
|June 5, 2018
PubMed
Summary

Performing platelet aggregometry with mouse models requires adapting standard methods due to differences in blood volume and platelet size. This review details essential considerations for successful mouse platelet assays, including sample handling and preparation techniques.

Keywords:
ADPOptimulPAR4PRPaggregometrymouseplatelet

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Area of Science:

  • Hematology
  • Platelet Biology
  • Translational Research

Background:

  • Light transmission aggregometry and lumi-aggregometry are standard platelet function assays.
  • Genetically modified mouse models are crucial for studying human diseases.
  • Adapting human platelet assay protocols for mouse platelets is necessary due to physiological differences.

Purpose of the Study:

  • To provide a comprehensive guide for performing platelet aggregometry with mouse platelets.
  • To highlight key methodological considerations for mouse platelet assays.
  • To discuss strategies for maximizing experimental throughput and data yield.

Main Methods:

  • Review of established and novel techniques for blood collection and anticoagulant use in mice.
  • Detailed protocols for optimizing mouse platelet isolation and preparation to maximize yield.
  • Exploration of high-throughput aggregometry methods and the use of thrombopoietin (TPO) mimetics.

Main Results:

  • Successful mouse platelet aggregometry requires careful attention to sample volume, anticoagulant choice, and platelet preparation.
  • Optimized methods can increase the number of assays performed per mouse.
  • Understanding mouse-specific platelet characteristics is vital for accurate interpretation.

Conclusions:

  • Standardized and adapted protocols are essential for reliable mouse platelet aggregometry.
  • Novel approaches like high-throughput assays and TPO-mimetics can enhance experimental efficiency.
  • This review serves as a critical resource for researchers utilizing mouse models in platelet research.