A comparative profiling of oral cancer patients and high risk niswar users using FT-IR and chemometric analysis

Oral cancer is one of the major cancer types, which has increased sustainably in Southeast Asian countries due to the extensive use of a variety of tobacco and betel nut products. The current study is focused on developing an easy, efficient and cost-effective method for plasma profiling of oral cancer patients and tobacco users in order to have a progressive picture towards oral cancer. For this purpose, the profiling of 147 plasma samples including 67 oral cancer patients' samples, 60 "niswar" (a dipping tobacco product) user samples, and 20 healthy controls using attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), and chemometric analysis was carried out. Fingerprint region (500-1500 cm^-1) of all three groups showed interesting variations in peaks pattern. From these observations, height ratios of two bands H1646/H1550 and H1080/H1024 with p value of 2.01 × 10^-6 and 8.39 × 10^-7, respectively, showed a pattern between healthy to oral cancer and "niswar" user samples. Chemometric analysis of the data showed a clean separation among the groups. PLS-DA and OPLS-DA models provided 87.7% and 89.5% classification rate, respectively. Area under the curve (AUC) for healthy control, oral cancer and "niswar" users were found to be 0.97, 0.95 and 0.92%, respectively. The results of the present study indicate that FT-IR spectroscopy, in conjunction with chemometric data, can be effectively used for the preliminary differentiation of plasma samples of oral cancer patients, "niswar" users and control samples of healthy persons.