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Published on: March 27, 2020
Two-Photon Fluorescent Probe for Monitoring Autophagy via Fluorescence Lifetime Imaging
Liling Hou1, Peng Ning1, Yan Feng1
1School of Chemistry and Chemical Engineering, Center for Atomic Engineering of Advanced Materials, and Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials , Anhui University , Hefei , Anhui 230601 , China.
Abstract:
We reported the first lysosome targeted two-photon fluorescent probe (Lyso-NP) as a viscosity probe for monitoring autophagy. The fluorescence lifetime of Lyso-NP exhibited an excellent linear relationship with viscosity value ( R2 = 0.99, x = 0.39). Lyso-NP also showed the specific capability for imaging lysosomal viscosity under two-photon excitation at 860 nm along with good biocompatibility. More importantly, Lyso-NP could be used to monitor the autophagy process in living cells by quantitatively detecting lysosomal viscosity changes during the membrane fusion process via two-photon fluorescence lifetime imaging.
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