Post-translational quantitation by SRM/MRM: applications in cardiology

Area of Science:

• Biochemistry
• Proteomics
• Molecular Biology

Background:

• Post-translational modifications (PTMs) are crucial for regulating protein function, localization, and interactions.
• PTMs provide dynamic control over proteins in both physiological and pathological states.
• Studying disease-specific PTMs aids in identifying biomarkers and developing targeted therapies.

Purpose of the Study:

• To review common PTMs, their biological roles, and detection methods.
• To highlight the application of Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) for absolute PTM quantitation.
• To focus on PTM detection in cardiovascular system proteins and heart diseases.

Main Methods:

• Enrichment techniques combined with high-resolution mass spectrometry (MS)/MS analysis for PTM characterization.
• Targeted proteomics using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS).
• Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) for quantitative validation of PTMs.

Main Results:

• SRM/MRM assays offer powerful quantitative analysis of PTMs in complex biological samples.
• LC-MS/MS has significantly advanced the understanding of PTMs in disease pathology.
• PTM characterization in cardiovascular proteins shows great potential for clinical applications.

Conclusions:

• Targeted proteomics, particularly SRM/MRM, is essential for PTM quantitation and validation.
• Advances in enrichment and software tools enhance the interpretation of complex PTM data.
• PTMomics holds significant promise for clinical perspectives, especially in cardiovascular research.