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Updated: Feb 9, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
Effects of Immunosenescence on the Lower Expression of Surface Molecules in Neutrophils and Lymphocytes
Alessandra B Lopes1, Larissa B Lopes2, Roseli N da Silveira Antunes3
1Postgraduate Program in Health and Aging, Marilia School of Medicine - FAMEMA, Rua Monte Carmelo, 800, Fragata, CEP: 17519-030, Marilia - SP, Brazil.
Background:
Immunosenescence is a remodeling of the immune system, caused by aging, with changes in the function of neutrophils, lymphocytes, and Treg cells.
Objective:
This study aimed to evaluate the expression of molecules CD11b, CD16 and CD64 (neutrophils), CD154 (T lymphocytes), CD40 (B lymphocytes), and to quantitatively analyze the Treg cell subpopulation.
Methods:
49 elderlies (≥60 years) and 49 adults (≤35 years) were studied. Flow cytometry was used to analyze the expression of surface molecules and the subpopulation of Treg cells, and the results between the groups were compared statistically by the t-test.
Results:
There was a decreased significance in the expression of CD11b and CD40 in the elderly.
Conclusion:
Decreased CD11b expression can result in susceptibility to infectious diseases, and impairment of phagocytic capacity. Decreased CD40 expression can result in a decline in B lymphocyte activation. The other molecule studied presented alterations not significant, but compatible with the immunological changes in aging.
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