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Related Experiment Videos

Specific binding sites for platelet activating factor in human lung tissues.

S B Hwang, M H Lam, T Y Shen

    Biochemical and Biophysical Research Communications
    |April 30, 1985
    PubMed
    Summary

    Human lung tissue has specific receptors for platelet-activating factor (PAF). Binding of [3H]-PAF to these receptors suggests a key role in PAF-induced lung inflammation.

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    Patents and literature.

    Applied biochemistry and biotechnology·2013

    Area of Science:

    • Immunology
    • Pharmacology
    • Cell Biology

    Background:

    • Platelet-activating factor (PAF) is a potent inflammatory mediator.
    • Understanding PAF receptor interactions is crucial for inflammatory lung disease research.

    Purpose of the Study:

    • To investigate the presence and characteristics of PAF receptors in human lung tissue.
    • To determine the binding affinity and specificity of PAF to its lung receptors.

    Main Methods:

    • Utilized radioligand binding assays with [3H]-labeled PAF.
    • Performed Scatchard analysis to quantify receptor binding parameters.
    • Tested the displacement of [3H]-PAF binding by known PAF receptor antagonists (kadsurenone, ginkgolide B).

    Main Results:

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    • Demonstrated specific and saturable binding of [3H]-PAF to human lung membranes.
    • Determined an equilibrium dissociation constant (KD) of 4.9 x 10(-10)M and maximal binding sites of 140 fmole/mg protein.
    • Observed similar selectivity for PAF analogs and displacement by antagonists as seen in rabbit platelets.

    Conclusions:

    • Human lung tissue possesses specific receptors for platelet-activating factor (PAF).
    • The characterized binding properties are consistent with a functional PAF receptor.
    • PAF receptor binding in the lungs is a potential initiating event in PAF-induced lung pathophysiology.