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Updated: Feb 9, 2026

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry
Published on: September 10, 2018
A Novel Method to Image Macropinocytosis in Vivo
Lunhao Chen1,2, Daxiao Cheng1, Jiachen Chu1,3
1Department of Neurobiology, Key Laboratory of Medical Neurobiology, Ministry of Health of China, Zhejiang Provincial Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China.
Abstract:
Here we described an experimental protocol for in vivo imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPγS into transparent Drosophila melanogaster embryos. Using a confocal microscope for live imaging, we monitored the generation of dextran-positive macropinosomes and subsequent intracellular events. Our protocol provides a continent and reliable way for investigating macropinocytosis and its underlying mechanisms, especially when combined with genetic strategies.
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