Combination Therapy Strategies Against Multiple-Resistant Streptococcus Suis
Yang Yu1,2,3,4, Jin-Tao Fang1,2,3, Mei Zheng3
1National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
Combination therapies of ampicillin plus apramycin and tiamulin plus spectinomycin show synergism against multi-drug resistant Streptococcus suis. These findings offer potential strategies for treating infections in animals and humans.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
- Zoonotic Diseases
Background:
- Streptococcus suis is a significant swine pathogen and emerging zoonotic agent.
- Antimicrobial resistance in S. suis poses a therapeutic challenge in both veterinary and human medicine.
- Multi-drug resistant S. suis strains necessitate novel treatment strategies.
Purpose of the Study:
- To investigate the synergistic effects of antibiotic combinations against multi-resistant Streptococcus suis isolates.
- To identify effective combination therapies for treating S. suis infections in swine.
- To evaluate the in vitro and in vivo efficacy of synergistic antibiotic combinations.
Main Methods:
- Antibiotic susceptibility profiling of 11 S. suis strains against 12 antibiotics.
- Checkerboard assays to determine synergistic and antagonistic activities of antibiotic combinations.
- In vitro killing curves and in vivo treatment trials to validate synergistic combinations.
Main Results:
- High resistance rates (80-100%) observed for erythromycin, clindamycin, trimethoprim/sulfamethoxazole, and tetracycline.
- Ampicillin plus apramycin and tiamulin plus spectinomycin demonstrated the highest synergistic activity.
- Combination therapies achieved >3-logCFU reduction in vitro, exceeding the <2-logCFU reduction by monotherapies.
- In vivo studies confirmed significant bacterial reduction with the identified combination regimens.
Conclusions:
- Ampicillin plus apramycin and tiamulin plus spectinomycin exhibit significant synergism against multi-resistant S. suis.
- These combination therapies represent promising strategies for managing S. suis infections in animals.
- Further research may explore their application in human medicine due to the zoonotic potential of S. suis.
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