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Related Experiment Video

Updated: Feb 9, 2026

Genome-wide Analysis of Aminoacylation Charging Levels of tRNA Using Microarrays

Genome-wide Analysis of Aminoacylation Charging Levels of tRNA Using Microarrays

Published on: June 18, 2010

Beyond the Limits: tRNA Array Units in Mycobacterium Genomes.

Sergio M Morgado¹, Ana C P Vicente¹

¹Laboratory of Molecular Genetics of Microorganisms, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Frontiers in Microbiology

|June 6, 2018

Summary

Transfer RNA (tRNA) array units, previously thought rare, are abundant in diverse Mycobacterium genomes, including chromosomes, phages, and plasmids. This finding expands our understanding of tRNA gene organization in bacteria.

Keywords:

HNH endonuclease bioinformatics code:Perl mycobacteria mycobacteria plasmids mycobacteriophages tRNA array units tmRNA and structured RNA

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Area of Science:

Genomics
Microbiology
Bioinformatics

Background:

Transfer RNA (tRNA) array units, regions with high tRNA gene density, were primarily identified in a few bacterial phyla like Firmicutes.
Their presence and organization in other bacterial genera, particularly Mycobacterium, remained largely unexplored.

Purpose of the Study:

To identify and characterize tRNA array units within genomes associated with the Mycobacterium genus.
To develop and apply a bioinformatics tool for detecting tRNA gene arrangement as array units.

Main Methods:

Development and application of an in-house Perl script for identifying tRNA array units.
Analysis of 7,670 Mycobacterium genomes, 4,312 mycobacteriophage genomes, and 40 mycobacteria plasmids.
Identification of other non-coding RNA species within these regions.

Main Results:

tRNA array units are abundant and diverse in Mycobacterium-associated genomes, including chromosomes, prophages, and plasmids.
These structures are particularly prevalent in Mycobacterium abscessus complex species.
Other non-coding RNAs, such as tmRNA and structured RNA, were also found in these regions.

Conclusions:

tRNA array units are not restricted to a few bacterial phyla, as previously assumed, but are widespread in diverse bacterial genera like Mycobacterium.
The presence of tRNA array units in mobile genetic elements (plasmids, bacteriophages) suggests a role in horizontal gene transfer and bacterial adaptation.
The developed bioinformatics tool facilitates further exploration of tRNA array units in large genomic datasets.