Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.7K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.7K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.9K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.9K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.0K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.0K
pre-mRNA Processing02:01

pre-mRNA Processing

57.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.5K
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small RNA sequencing reveals drought-responsive microRNAs and regulatory networks in roots of mungbean genotypes differing in drought tolerance.

BMC plant biology·2026
Same author

Metagenomic mining of microbial communication genes from Indian deep-sea sediments using a quorum sensing- and quenching-related protein database.

Marine genomics·2026
Same author

Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.

BMC plant biology·2026
Same author

Modern Approaches in Genetic Toxicology Research: Insights from Next-Generation-Based RNA Sequencing.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Guest Editorial: Special Issue: "Biology of Plant Viruses and the Resistance Strategies of the Plant Hosts".

Physiology and molecular biology of plants : an international journal of functional plant biology·2025
Same author

Utility of combined solid and liquid biopsy for molecular profiling in lung adenocarcinoma: Insights from a real-world case.

The journal of liquid biopsy·2025

Related Experiment Video

Updated: Feb 9, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K

ARMOUR - A Rice miRNA: mRNA Interaction Resource.

Neeti Sanan-Mishra1, Anita Tripathi1, Kavita Goswami1

  • 1Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.

Frontiers in Plant Science
|June 6, 2018
PubMed
Summary

ARMOUR is a new database detailing rice microRNA (miRNA) and messenger RNA (mRNA) interactions. It provides expression profiles for known and novel miRNAs under various conditions, aiding research in rice molecular biology.

Keywords:
databaseexpressiongene annotationmiRNApathwaytarget

More Related Videos

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.6K
Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
11:21

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

4.1K

Related Experiment Videos

Last Updated: Feb 9, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.6K
Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
11:21

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

4.1K

Area of Science:

  • Plant Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression in plants.
  • Understanding miRNA:mRNA interactions is crucial for elucidating cellular machinery regulation in rice.
  • Existing resources may lack comprehensive, experimentally validated data on rice miRNA expression and interactions.

Purpose of the Study:

  • To develop ARMOUR, an interactive database for rice miRNA:mRNA interactions.
  • To provide experimentally validated expression profiles of miRNAs across diverse conditions and rice cultivars.
  • To facilitate the exploration of miRNA targets, functional annotations, and pathway involvement.

Main Methods:

  • Database development integrating known and predicted novel miRNAs.
  • Inclusion of experimentally validated miRNA expression profiles across 38+ tissues/conditions.
  • Implementation of flexible querying options including gene identifiers, GO, KEGG, and BLAST search.

Main Results:

  • ARMOUR contains 689 known and 1664 predicted novel miRNAs.
  • Expression profiles are available for multiple Indian rice cultivars under developmental and abiotic stress conditions.
  • The database links miRNAs to their predicted/known target transcripts, GO annotations, and pathways.

Conclusions:

  • ARMOUR serves as a valuable, cohesive resource for rice miRNA research.
  • The database supports the investigation of miRNA:mRNA interactome in rice functional genomics.
  • ARMOUR encourages data sharing and comparison among rice research groups.