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A guideline for reporting experimental protocols in life sciences.

Olga Giraldo1, Alexander Garcia1,2, Oscar Corcho1

  • 1Ontology Engineering Group, Campus de Montegancedo, Boadilla del Monte, Universidad Politécnica de Madrid, Madrid, Spain.

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|June 6, 2018
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Summary
This summary is machine-generated.

This study introduces a guideline and checklist for reporting experimental protocols in life sciences. It ensures sufficient detail for reproducibility and consistency in research methods.

Keywords:
ChecklistExperimental protocolsGood practices for reporting protocolsGuidelinesOpen scienceRecommendationsReproducibility

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Area of Science:

  • Life Sciences
  • Biotechnology
  • Molecular Biology

Background:

  • Experimental protocols are crucial for research reproducibility but vary in content and structure.
  • Standardized reporting of methods is essential for scientific integrity.

Purpose of the Study:

  • To present a guideline for describing key content in life science experimental protocols.
  • To develop a checklist of fundamental data elements for protocol execution and reporting.

Main Methods:

  • Development of a guideline for experimental protocol reporting.
  • Creation of a checklist with 17 fundamental data elements.
  • Formal description of data elements using the SMART Protocols ontology.

Main Results:

  • A proposed checklist of 17 essential data elements for experimental protocols.
  • Formalized data elements within the SMART Protocols ontology.
  • Guidance focused on the content required for protocol execution.

Conclusions:

  • The guideline and checklist aim to improve the reporting of experimental protocols in life sciences.
  • Enhanced reporting facilitates experiment reproducibility and promotes inter-laboratory consistency.
  • The checklist provides criteria for evaluating the quality of reported experimental methods.