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Updated: Feb 9, 2026
11:37
Cellular Redox Profiling Using High-content Microscopy
Published on: May 14, 2017
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Temporal profiling of redox-dependent heterogeneity in single cells.
Meytal Radzinski1, Rosi Fassler1, Ohad Yogev1
1Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, The Hebrew University of Jerusalem, Jerusalem, Israel.
Elife
|June 6, 2018
Summary
Researchers developed a new method to track cellular redox status in single yeast cells. This revealed distinct subpopulations with varying oxidation levels, impacting cellular functions and identifying key proteins for redox homeostasis.
Area of Science:
- Cellular Biology
- Biochemistry
- Genetics
Background:
- Cellular redox status is crucial for fundamental processes like proliferation, protein maintenance, and aging.
- Variations in redox status can create distinct cell subpopulations, even within genetically identical populations.
Purpose of the Study:
- To develop a novel method for tracking redox status at the single-cell level.
- To identify and characterize redox-dependent heterogeneity in yeast cell populations.
- To investigate the proteomic and transcriptomic profiles of these distinct redox subpopulations.
Main Methods:
- Utilized a novel methodology employing the redox-sensitive probe Grx1-roGFP2.
- Enabled identification and sorting of subpopulations based on cytosolic, mitochondrial, or peroxisomal oxidation levels.
- Performed comparative proteomic and transcriptomic analyses of sorted redox subpopulations.
Main Results:
- Defined redox-dependent heterogeneity in yeast, revealing bi-modal oxidation status distribution in late logarithmic growth phase.
- Characterized growth, proteomic, and transcriptomic profiles of distinct redox subpopulations.
- Identified Hsp30, Dhh1, and Pnc1 as key proteins influencing basal oxidation levels.
Conclusions:
- The novel method allows for high-resolution analysis of cellular redox status and heterogeneity.
- Specific proteins (Hsp30, Dhh1, Pnc1) play a significant role in maintaining redox homeostasis.
- Understanding redox heterogeneity is vital for comprehending cellular function and aging.
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