Long noncoding RNA FAM83H-AS1 exerts an oncogenic role in glioma through epigenetically silencing CDKN1A (p21)

Related Experiment Video

Updated: Feb 9, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.9K

Yong-Yan Bi1, Gang Shen1, Yong Quan1

  • 1Department of Neurosurgery, Minhang Hospital, Fudan University, Shanghai, China.

Journal of Cellular Physiology
|June 6, 2018
PubMed

Insights

FAM83H-AS1, a long noncoding RNA, is upregulated in glioma, promoting tumor growth by silencing CDKN1A. Silencing FAM83H-AS1 suppresses glioma progression, suggesting it as a therapeutic target.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Gliomas are aggressive primary central nervous system tumors.
  • Long noncoding RNAs (lncRNAs) play roles in tumorigenesis.
  • FAM83H-AS1 is dysregulated in various cancers, but its role in glioma is unclear.

Purpose of the Study:

  • Investigate the biological role of FAM83H-AS1 in glioma.
  • Determine if FAM83H-AS1 can serve as a prognostic biomarker or therapeutic target for glioma.

Main Methods:

  • Analysis of FAM83H-AS1 expression in glioma tissues and cell lines.
  • Loss-of-function assays to assess the impact of FAM83H-AS1 silencing on cell proliferation and apoptosis.
  • Mechanistic studies to elucidate the molecular pathways involving FAM83H-AS1, EZH2, and CDKN1A.

Main Results:

  • FAM83H-AS1 expression is upregulated in glioma tissues and cell lines.
  • High FAM83H-AS1 levels correlate with poor glioma prognosis.
  • Silencing FAM83H-AS1 suppresses glioma cell proliferation by regulating cell cycle and apoptosis.
  • FAM83H-AS1 recruits EZH2 to silence CDKN1A expression, impacting cell cycle and proliferation.

Conclusions:

  • FAM83H-AS1 promotes glioma progression.
  • FAM83H-AS1 acts as a potential therapeutic target and prognostic biomarker for human glioma.
Keywords:
CDKN1AEZH2FAM83H-AS1H3K27me3glioma

More Related Videos

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

9.3K
Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data07:35

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

Published on: December 1, 2023

1.2K

Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.9K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.2K
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.0K