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Designing Silk-silk Protein Alloy Materials for Biomedical Applications
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The effects of proteins released from silk mat layers on macrophages

Ju-Won Kim1, You-Young Jo2, Hae Yong Kweon2

  • 11Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University, Jukheon gil 7, Gangneung, Gangwondo 25457 Republic of Korea.

Maxillofacial Plastic and Reconstructive Surgery
|June 7, 2018
PubMed
Abstract

Insights

Proteins from the outer and inner layers of silkworm cocoons significantly alter gene expression related to inflammation and angiogenesis. Middle cocoon layers release fewer proteins and have minimal impact on these key biological processes.

Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Cell Biology

Background:

  • Silk cocoons from *Bombyx mori* are composed of distinct layers.
  • Proteins released from these layers may influence cellular behavior.
  • Understanding layer-specific protein effects is crucial for silk-based applications.

Purpose of the Study:

  • To investigate gene expression changes in cells exposed to proteins from different silk cocoon layers.
  • To determine if protein concentration and biological activity vary across silk mat layers.

Main Methods:

  • Silk cocoon layers (1-4, inner to outer) were isolated.
  • Proteins were extracted via sonication and quantified.
  • RAW264.7 cells were incubated with extracted proteins.
  • Gene expression was analyzed using cDNA microarray and qRT-PCR.

Main Results:

  • Higher protein concentrations were found in layers 1 and 4 compared to layers 2 and 3.
  • Significantly elevated expression of inflammation and angiogenesis-associated genes occurred in layer 1 and 4 groups.
  • qRT-PCR results confirmed cDNA microarray findings.

Conclusions:

  • Proteins from the inner (1) and outer (4) silk cocoon layers induce notable changes in inflammation and angiogenesis gene expression.
  • Proteins from the middle layers (2 and 3) showed lower release and less impact on these genes.
  • This suggests layer-specific properties of silk proteins influence cellular responses.
Keywords:
Angiogenesis inducing agentsInflammationSilk

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