Bicarbonate directly modulates activity of chemosensitive neurons in the retrotrapezoid nucleus

Changes in CO₂ result in corresponding changes in both H⁺ and HCO₃^- and despite evidence that HCO₃^- can function as an independent signalling molecule, there is little evidence suggesting HCO₃^- contributes to respiratory chemoreception. We show that HCO₃^- directly activates chemosensitive retrotrapezoid nucleus (RTN) neurons. Identifying all relevant signalling molecules is essential for understanding how chemoreceptors function, and because HCO₃^- and H⁺ are buffered by separate cellular mechanisms, having the ability to sense both modalities adds additional information regarding changes in CO₂ that are not necessarily reflected by pH alone. HCO₃^- may be particularly important for regulating activity of RTN chemoreceptors during sustained intracellular acidifications when TASK-2 channels, which appear to be the sole intracellular pH sensor, are minimally active.

Central chemoreception is the mechanism by which the brain regulates breathing in response to changes in tissue CO₂ /H⁺ . The retrotrapezoid nucleus (RTN) is an important site of respiratory chemoreception. Mechanisms underlying RTN chemoreception involve H⁺ -mediated activation of chemosensitive neurons and CO₂ /H⁺ -evoked ATP-purinergic signalling by local astrocytes, which activates chemosensitive neurons directly and indirectly by maintaining vascular tone when CO₂ /H⁺ levels are high. Although changes in CO₂ result in corresponding changes in both H⁺ and HCO₃^- and despite evidence that HCO₃^- can function as an independent signalling molecule, there is little evidence suggesting HCO₃^- contributes to respiratory chemoreception. Therefore, the goal of this study was to determine whether HCO₃^- regulates activity of chemosensitive RTN neurons independent of pH. Cell-attached recordings were used to monitor activity of chemosensitive RTN neurons in brainstem slices (300 μm thick) isolated from rat pups (postnatal days 7-11) during exposure to low or high concentrations of HCO₃^- . In a subset of experiments, we also included 2',7'-bis(2carboxyethyl)-5-(and 6)-carboxyfluorescein (BCECF) in the internal solution to measure pHi under each experimental condition. We found that HCO₃^- activates chemosensitive RTN neurons by mechanisms independent of intracellular or extracellular pH, glutamate, GABA, glycine or purinergic signalling, soluble adenylyl cyclase activity, nitric oxide or KCNQ channels. These results establish HCO₃^- as a novel independent modulator of chemoreceptor activity, and because the levels of HCO₃^- along with H⁺ are buffered by independent cellular mechanisms, these results suggest HCO₃^- chemoreception adds additional information regarding changes in CO₂ that are not necessarily reflected by pH.