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MRC-5 cells, a model for Junín virus persistent infection.
The Journal of General Virology
|May 1, 1985
Summary
Junín virus (JV) readily establishes persistent infections in MRC-5 cells, a diploid cell line. This model is suitable for studying viral persistence and potential alterations in virus properties.
Area of Science:
- Virology
- Cell Biology
Background:
- Junín virus (JV) is an arenavirus known to cause Argentine hemorrhagic fever.
- Persistent viral infections can alter host cell characteristics and viral properties.
Purpose of the Study:
- To establish and characterize persistent Junín virus infections in MRC-5 cells.
- To evaluate MRC-5 cells as a model for studying JV persistence.
Main Methods:
- Inoculation of MRC-5 cells with attenuated Junín virus strains.
- Monitoring of viral replication, cytopathic effects, and viral release.
- Analysis of viral properties in persistently infected cells.
Main Results:
- Persistent Junín virus infection was commonly established in MRC-5 cells, independent of viral strain or multiplicity of infection.
- Persistently infected cells released infectious virus throughout their lifespan.
- Altered viral properties, including plaque morphology and temperature sensitivity, were observed, suggesting viral adaptation.
Conclusions:
- MRC-5 cells provide a suitable diploid cell model for studying Junín virus persistence.
- Persistent JV infection in MRC-5 cells can lead to alterations in viral characteristics.
- Further research into JV persistence mechanisms and implications is warranted.