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Short-Term Hyperprolactinemia Reduces the Expression of Purinergic P2X7 Receptors during Allergic Inflammatory

Julieta E Ochoa-Amaya1,2, Nicolle Queiroz-Hazarbassanov1, Lilian B Namazu1

  • 1Departamento de Patologia da Faculdade de Medicina Veterinária da Universidade de São Paulo, São Paulo, Brazil.

Neuroimmunomodulation
|June 7, 2018
PubMed
Abstract

Insights

Short-term hyperprolactinemia, induced by domperidone, reduces allergic lung inflammation by altering prolactin and P2X7 receptors. This suggests a novel therapeutic pathway for allergic respiratory conditions.

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Endocrinology

Background:

  • Domperidone-induced hyperprolactinemia has been shown to reduce allergic lung inflammation.
  • Purinergic receptor P2X7R activity is linked to proinflammatory cytokine release and allergic respiratory disease pathogenesis.

Purpose of the Study:

  • To investigate the involvement of purinergic and prolactin receptors in the anti-allergic effects of hyperprolactinemia.
  • To explore the role of P2X7R and prolactin receptors in domperidone-induced hyperprolactinemia's impact on allergic lung inflammation.

Main Methods:

  • Hyperprolactinemia was induced in rats via daily intraperitoneal injections of domperidone.
  • Lung P2X7 receptor expression was assessed using immunohistochemistry.
  • Prolactin receptor expression in bronchoalveolar lavage leukocytes was analyzed by flow cytometry.

Main Results:

  • Hyperprolactinemia increased prolactin receptor expression in granulocytes.
  • Allergic conditions showed increased P2X7 receptor expression, which was significantly reduced by hyperprolactinemia.
  • Leukocyte counts, particularly granulocytes, in bronchoalveolar lavage decreased under hyperprolactinemia.

Conclusions:

  • Changes in both purinergic and prolactin receptor expression are associated with the anti-asthmatic effect of hyperprolactinemia.
  • These receptor alterations may mediate the reduction in allergic lung inflammation observed in hyperprolactinemic states.
Keywords:
AsthmaDomperidoneLungProlactinPurinergic receptor

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