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Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
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Study of Azole - Resistant and Cyp51a Gene in Aspergillus Fumigatus

Majid Zarrin1, Sama Faramarzi2

  • 1Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Open Access Macedonian Journal of Medical Sciences
|June 8, 2018
PubMed
Abstract

Insights

Azole resistance was investigated in Aspergillus fumigatus. Voriconazole resistance was detected in 15.5% of isolates, with no mutations found in the cyp51A gene.

Area of Science:

  • Mycology
  • Antimicrobial Resistance
  • Molecular Biology

Background:

  • Aspergillus fumigatus is a significant fungal pathogen.
  • Azole antifungals are crucial for treating invasive aspergillosis.
  • Emergence of azole resistance in A. fumigatus poses a clinical challenge.

Purpose of the Study:

  • To determine the prevalence of azole resistance in Aspergillus fumigatus isolates.
  • To perform molecular analysis of the cyp51A gene in resistant isolates.

Main Methods:

  • Fifty-eight A. fumigatus strains (environmental, clinical, reference) were analyzed.
  • Azole susceptibility testing for itraconazole and voriconazole was conducted.
  • Polymerase chain reaction (PCR) amplified the cyp51A gene for sequencing.

Main Results:

  • Voriconazole resistance was identified in 9 (15.5%) isolates (MIC = 4 µg/ml).
  • Itraconazole MICs ranged from 0.125 to 2 µg/ml.
  • Sequencing of the amplified cyp51A gene fragments showed 99% identity to A. fumigatus, with no point mutations detected.

Conclusions:

  • Voriconazole resistance was confirmed in the studied A. fumigatus isolates.
  • The cyp51A gene did not show mutations in resistant strains, suggesting other resistance mechanisms may be involved.
  • Antifungal susceptibility testing is recommended for patients infected with A. fumigatus.
Keywords:
Aspergillus fumigatusAzole-resistantCyp51 A gene

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