Related Experiment Video
Updated: Feb 9, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Isolation, characterization and antibacterial effect of biosurfactant from Candida parapsilosis
Mayank Garg1, Priyanka1, Mary Chatterjee1
1Biotechnology Engineering, U.I.E.T., Panjab University, Chandigarh, India.
Abstract:
In the present study, a biosurfactant producing Candida parapsilosis strain was isolated and identified by our laboratory. Different biosurfactant screening tests such as drop collapse, oil spreading, emulsification index and hemolytic activity confirmed the production of biosurfactant by the isolated Candida parapsilosis strain. The biosurfactant showed significant emulsifying index, drop collapse and oil-spread activity. The partially purified biosurfactant was characterized by Fourier Transform Infrared Spectroscopy (FT-IR) and Gas Chromatography-Mass Spectroscopy (GC-MS). The FT-IR results indicated phenol (O-H), amide (N-H) and carbon functional group peaks like C[bond, double bond]O and C[bond, double bond]C at their identified places. GC-MS analysis revealed the presence of 13-docosenamide type of compound with a molecular weight of 337.5 g mol-1. The isolated biosurfactant showed significant antibacterial activity against pathogenic Escherichia coli and Staphylococcus aureus strains at the concentrations of 10 and 5 mg ml-1 respectively. Growth inhibition of both Gram positive and Gram negative pathogenic strains designated the future prospect of exploring the isolated biosurfactant as broad spectrum antibacterial agent.
Related Concept Videos
DNA Isolation
DNA Isolation
Overview Of Cell Separation And Isolation
Cyclic Processes And Isolated Systems
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
Techniques for Isolation of Pure Cultures
Reduced Mass Coordinates: Isolated Two-body Problem

