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Data on genome sequencing, analysis and annotation of a pathogenic Bacillus cereus 062011msu

Rashmi Rathy1, Sayan Paul1, Vasanthakumar Ponesakki1

  • 1Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu 627012, India.

Data in Brief
|June 8, 2018
PubMed

Insights

A harmful Bacillus strain causing abscesses in sheep and goats was identified as Bacillus cereus 062011msu through whole genome sequencing. This pathogenic bacterium impacts livestock production, necessitating further study.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Pathogen Identification

Background:

  • A pathogenic Bacillus strain, identified as 062011 msu, causes abscesses in sheep and goats, impacting livestock productivity.
  • This strain specifically affects female animals, leading to decreased milk and meat yields.

Purpose of the Study:

  • To perform whole genome sequencing of the pathogenic Bacillus isolate 062011 msu.
  • To analyze the genome for genetic elements, metabolic pathways, virulence factors, and antibiotic resistance genes.
  • To accurately identify and reclassify the bacterial strain based on genomic data.

Main Methods:

  • Whole genome sequencing using the Ion Torrent platform.
  • Genome assembly and annotation.
  • Comparative genomics and 16S rRNA sequencing.

Main Results:

  • Generated 458,944 raw reads with an average length of 198.2 bp.
  • Confirmed the strain belongs to the Bacillus cereus species with 99% homology to known B. cereus genomes.
  • Identified genetic islands, metabolic pathways, virulence factors, and antibiotic resistance genes.

Conclusions:

  • The pathogenic strain 062011 msu is reclassified as Bacillus cereus 062011msu.
  • The genomic data provides insights into the pathogenicity and potential control strategies for this livestock pathogen.
Keywords:
AbscessationBacillus cereusGenome sequencingVirulence factors

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