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Fluorescence microscopy data on expression of Paired Box Transcription Factor 7 in skeletal muscle of APOBEC2

Hideaki Ohtsubo1, Yusuke Sato1,2, Yuji Matsuyoshi1

  • 1Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.

Data in Brief
|June 8, 2018
PubMed

Insights

APOBEC2 plays a crucial role in muscle regeneration by supporting the self-renewal of myogenic stem satellite cells. Its absence reduces the number of Pax7-positive cells, impacting muscle repair.

Area of Science:

  • Muscle regeneration
  • Cellular biology
  • Biochemistry

Background:

  • Myoblast differentiation is key to muscle regeneration.
  • Myogenic stem satellite cells are crucial for muscle repair and self-renewal.
  • APOBEC2's role in this process requires further elucidation.

Purpose of the Study:

  • To investigate the in vivo role of APOBEC2 in muscle regeneration.
  • To determine APOBEC2's effect on myogenic stem satellite cell self-renewal.
  • To understand APOBEC2's regulation of myoblast differentiation.

Main Methods:

  • In vivo phenotypic analysis in mice.
  • Comparison of APOBEC2-knockout and wild-type muscle tissues.
  • Quantification of Paired Box Transcription Factor 7 (Pax7)-positive cells per myofiber.

Main Results:

  • APOBEC2-knockout muscle showed a significantly lower number of Pax7-positive cells compared to wild-type.
  • This reduction was observed in 8-week-old mice.
  • Data suggest APOBEC2 is essential for maintaining the quiescent state of satellite cells post-activation.

Conclusions:

  • APOBEC2 is vital for the self-renewal of myogenic stem satellite cells.
  • APOBEC2 negatively regulates myoblast differentiation.
  • The findings highlight APOBEC2's importance in muscle regeneration and stem cell function.
Keywords:
APOBEC2Cryo-sectionsImmunofluorescenceMuscleMyogenic stem satellite cellsSelf-renewal

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