Related Experiment Video
Updated: Feb 9, 2026
05:33
Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
3.9K
Hymenobacter segetis sp. nov., isolated from soil.
Leonid N Ten1, Soo Jeong Lim2, Byung-Oh Kim2
1School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Archives of Microbiology
|June 8, 2018
Summary
A novel bacterium, Hymenobacter segetis sp. nov., was identified from Korean soil. This new species, strain S7-3-11T, expands the known diversity within the Hymenobacter genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Hymenobacter comprises diverse bacteria found in various environments.
- Accurate taxonomic classification is crucial for understanding microbial diversity and ecological roles.
Purpose of the Study:
- To characterize a novel bacterial isolate from soil in the Republic of Korea.
- To determine the taxonomic position of the novel strain S7-3-11T within the genus Hymenobacter.
Main Methods:
- Polyphasic taxonomic analysis including 16S rRNA gene sequencing.
- DNA-DNA hybridization to assess genomic relatedness.
- Analysis of chemotaxonomic data (fatty acids, polar lipids, respiratory quinones).
Main Results:
- Strain S7-3-11T showed high 16S rRNA gene sequence similarity to known Hymenobacter species but low DNA-DNA hybridization values.
- Chemotaxonomic analyses revealed distinct profiles, including major fatty acids and polar lipids.
- The DNA G+C content was determined to be 60.2 mol%.
Conclusions:
- Strain S7-3-11T represents a distinct genospecies within the genus Hymenobacter.
- The novel species is named Hymenobacter segetis sp. nov., with strain S7-3-11T as the type strain.
Related Concept Videos
02:23The Soil Ecosystem
24.9K
Plants obtain inorganic minerals and water from the soil, which acts as a natural medium for land plants. The composition and quality of soil depend not only on the chemical constituents but also on the presence of living organisms. In general, soils contain three major components:
24.9K
01:24DNA Isolation
45.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
45.2K
01:34DNA Isolation
199.8K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
199.8K
01:20Overview Of Cell Separation And Isolation
7.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.6K
01:19Cyclic Processes And Isolated Systems
3.5K
A thermodynamic system with zero heat exchange and work is an isolated system. For these systems, the internal energy remains constant.
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
3.5K
01:24Techniques for Isolation of Pure Cultures
2.7K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
2.7K