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Published on: March 22, 2024
TUP1-mediated filamentation in Candida albicans leads to inability to colonize the mouse gut
Elvira Román1, Blanca Huertas1, Daniel Prieto1
1Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, E-28040 Madrid, Spain.
Aim:
To investigate the role of Candida albicans TUP1-mediated filamentation in the colonization of the mice gut.
Materials & Methods:
We used molecular genetics to generate a strain where filamentation is regulated by altering the expression of the TUP1 gene with tetracyclines.
Results:
The colonization rates reached with the TUP1REP-RFPREP strain were lower compared with wild-type strain and completely absent after induction of filamentation. No differences in the susceptibility to bile salts nor in the adhesion to the mouse intestine epithelium were observed.
Conclusion:
Blockage of C. albicans in a filamentous form impedes gut cell colonization in the mouse.
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