Experimental study on the sensitive depth of backwards detected light in turbid media

In the recent past, optical spectroscopy and imaging methods for biomedical diagnosis and target enhancing have been widely researched. The challenge to improve the performance of these methods is to know the sensitive depth of the backwards detected light well. Former research mainly employed a Monte Carlo method to run simulations to statistically describe the light sensitive depth. An experimental method for investigating the sensitive depth was developed and is presented here. An absorption plate was employed to remove all the light that may have travelled deeper than the plate, leaving only the light which cannot reach the plate. By measuring the received backwards light intensity and the depth between the probe and the plate, the light intensity distribution along the depth dimension can be achieved. The depth with the maximum light intensity was recorded as the sensitive depth. The experimental results showed that the maximum light intensity was nearly the same in a short depth range. It could be deduced that the sensitive depth was a range, rather than a single depth. This sensitive depth range as well as its central depth increased consistently with the increasing source-detection distance. Relationships between sensitive depth and optical properties were also investigated. It also showed that the reduced scattering coefficient affects the central sensitive depth and the range of the sensitive depth more than the absorption coefficient, so they cannot be simply added as reduced distinct coefficients to describe the sensitive depth. This study provides an efficient method for investigation of sensitive depth. It may facilitate the development of spectroscopy and imaging techniques for biomedical diagnosis and underwater imaging.