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Updated: Feb 9, 2026

In Vitro Transcription Assays and Their Application in Drug Discovery
Published on: September 20, 2016
Assay Guidance Manual: Quantitative Biology and Pharmacology in Preclinical Drug Discovery
Nathan P Coussens1, G Sitta Sittampalam1, Rajarshi Guha1
1National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, United States.
Abstract:
The Assay Guidance Manual (AGM) is an eBook of best practices for the design, development, and implementation of robust assays for early drug discovery. Initiated by pharmaceutical company scientists, the manual provides guidance for designing a "testing funnel" of assays to identify genuine hits using high-throughput screening (HTS) and advancing them through preclinical development. Combined with a workshop/tutorial component, the overall goal of the AGM is to provide a valuable resource for training translational scientists.
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