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Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Published on: August 24, 2015
Quality control of riboflavin-treated platelet concentrates using Mirasol® PRT system: Polish experience
Elżbieta Lachert1, Jolanta Kubis1, Jolanta Antoniewicz-Papis1
1Institute of Hematology and Transfusion Medicine, Warszawa, Poland.
Background:
The quality of platelet concentrates (PCs) is affected by preparation, storage, the type of container, and pathogen reduction technology (PRT). The Mirasol® Pathogen Reduction Technology (PRT) system (Terumo BCT Inc., Lakewood, USA), which uses riboflavin and ultraviolet (UV) light, has recently been proven effective against bacteria, viruses, parasites, and leukocytes.
Objectives:
The aim of the study was to evaluate the effect of the Mirasol® PRT system, based on riboflavin and UV light exposure, on the most common in vitro platelet quality parameters of PCs prepared from whole blood-derived buffy coats.
Material And Methods:
The study included 15 trials (n = 15). For each trial, 2 PCs were used: 1 for treatment with the Mirasol® PRT system (M) and 1 for a control (C). In the M group, PCs were illuminated. In the C group, saline solution was added. PCs from groups M and C were stored at 20-24°C, with agitation. Samples were collected on days 1, 3 and 5 to determine platelet concentration, total platelet count/unit, mean platelet volume (MPV), power of hydrogen (pH), glucose and beta-thromboglobulin concentration (BTG), hypotonic shock response (HSR), aggregation, CD42b and CD62P expression, pCO2, and pO2.
Results:
No significant differences in HSR or CD42b expression were observed between groups M and C. All pH values were stable during the whole storage period (7.1-7.5). On storage day 1, CD62P expression in group C was significantly higher than in group M. In the Mirasol® group, significantly higher glucose consumption was noted on storage days 3 and 5. On day 5, a 2-3-fold increase in BTG was observed in both groups as compared to day 1; on day 5, BTG concentration was 32% higher in group M than in group C. On all storage days, pCO2 was comparable in groups M and C; lower pO2 values were reported for group M.
Conclusions:
In vitro results demonstrated that pH, HSR, aggregation, CD42b antigen expression, and MPV and platelet count parameters were comparable in groups M and C.
Insights
The Mirasol® Pathogen Reduction Technology (PRT) system effectively maintains platelet concentrate (PC) quality, showing comparable results to controls for key parameters like pH and platelet count. This PRT system offers a viable option for pathogen-inactivated platelet storage.
Area of Science:
- Transfusion Medicine
- Hematology
- Biotechnology
Background:
- Platelet concentrate (PC) quality is influenced by preparation, storage, containers, and pathogen reduction technologies (PRT).
- The Mirasol® PRT system utilizes riboflavin and UV light, demonstrating efficacy against various pathogens and leukocytes.
Purpose of the Study:
- To assess the impact of the Mirasol® PRT system on in vitro platelet quality parameters.
- To evaluate PCs derived from whole blood-derived buffy coats after Mirasol® treatment.
Main Methods:
- 15 trials were conducted, comparing Mirasol® PRT-treated PCs (M) with control PCs (C).
- PCs were stored at 20-24°C with agitation.
- Quality parameters including platelet concentration, MPV, pH, glucose, BTG, HSR, aggregation, CD42b/CD62P expression, pCO2, and pO2 were measured on days 1, 3, and 5.
Main Results:
- No significant differences were found in hypotonic shock response (HSR) or CD42b expression between groups.
- pH remained stable (7.1-7.5) throughout storage.
- CD62P expression was lower in the Mirasol® group on day 1.
- Higher glucose consumption and increased beta-thromboglobulin (BTG) were observed in the Mirasol® group on later storage days.
- pCO2 levels were comparable, while pO2 was lower in the Mirasol® group.
Conclusions:
- The Mirasol® PRT system did not significantly alter key in vitro platelet quality parameters.
- Parameters such as pH, HSR, aggregation, CD42b expression, MPV, and platelet count were comparable between treated and control groups.
- The study indicates the Mirasol® system's potential for maintaining platelet quality during storage.
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