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Complete sequence of IS3.

K P Timmerman, C P Tu

    Nucleic Acids Research
    |March 25, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers sequenced IS3, revealing a 1258 bp element with inverted repeats and potential for bicistronic mRNA. This transposon shows limited homology to other elements but shares conserved features with IS2 transposases.

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    Area of Science:

    • Molecular Biology
    • Genomics
    • Microbial Genetics

    Background:

    • Transposable elements, such as insertion sequences (IS), play crucial roles in genome evolution and regulation.
    • Understanding the structure and function of novel IS elements is essential for deciphering their impact on host organisms.

    Purpose of the Study:

    • To determine the complete nucleotide sequence of the IS3 insertion sequence.
    • To analyze the structural features and potential coding capacity of IS3.
    • To investigate evolutionary relationships between IS3 and other known IS elements.

    Main Methods:

    • DNA sequencing to determine the nucleotide composition of IS3.
    • Bioinformatic analysis to identify open reading frames (ORFs) and regulatory signals.
    • Sequence homology searches (DNA and amino acid) against existing databases.

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    Main Results:

    • The IS3 sequence is 1258 bp long, featuring 39 bp inverted repeats with 6 mismatches.
    • IS3 contains a large 288-codon ORF and a smaller, overlapping 91-codon ORF on the opposite strand.
    • A 99-codon ORF with potential transcriptional and translational start signals precedes the large ORF, suggesting bicistronic mRNA encoding.
    • No significant DNA homology was found with other prokaryotic IS elements, but slight amino acid homology and conserved hydropathicity patterns exist with IS2 transposases.

    Conclusions:

    • IS3 possesses a complex genetic organization with the potential to encode multiple proteins via a bicistronic mRNA.
    • The regulatory mechanisms, including potential sequestration of Shine-Dalgarno sequences, may prevent fortuitous activation by external promoters.
    • IS3 represents a distinct IS element, with evolutionary links suggested by conserved protein features rather than extensive DNA sequence similarity.