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Related Experiment Videos

Junction sequences generated by 'one-ended transposition'.

S Mötsch, R Schmitt, P Avila

    Nucleic Acids Research
    |May 10, 1985
    PubMed
    Summary
    This summary is machine-generated.

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    One-ended transposition of Tn21 and Tn1721 elements involves plasmid fusion. The mechanism closely resembles the transposition of entire elements, with specific DNA sequence characteristics observed in the recombinants.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Transposable elements like Tn21 and Tn1721 play crucial roles in bacterial genome evolution.
    • Understanding their transposition mechanisms is key to comprehending genetic exchange and adaptation.

    Purpose of the Study:

    • To investigate the mechanism of 'one-ended transposition' involving single inverted repeat (IR) sequences of Tn21 and Tn1721.
    • To characterize the DNA junctions in recombinant molecules formed during this process.

    Main Methods:

    • Analysis of DNA junctions in plasmids after one-ended transposition.
    • Comparison of recombinant characteristics with those of entire element transposition.

    Main Results:

    • One-ended transposition results in plasmid fusion mediated by the cognate transposase.

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  • Donor DNA segment starts precisely at the IR sequence and is flanked by direct repeats of host DNA.
  • Recombinant characteristics are similar to those generated by entire Tn21 and Tn1721 transposition.

    • Conclusions:

    • The mechanism of one-ended transposition is highly similar to the transposition of the complete Tn21 and Tn1721 elements.
    • This finding provides insights into the flexibility and conserved mechanisms of transposon activity.