Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rhizobium meliloti carries two megaplasmids.

Z Banfalvi, E Kondorosi, A Kondorosi

    Plasmid
    |March 1, 1985
    PubMed
    Summary

    Rhizobium meliloti strain 41 harbors two megaplasmids, pRme41b and pRme41c. Researchers found these megaplasmids can transfer to other bacteria, with pRme41b containing genes for surface exclusion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Transformation of floral organs with GFP in Medicago truncatula.

    Plant cell reports·2019
    Same author

    Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp. falcata lines improved in somatic embryogenesis.

    Plant cell reports·2019
    Same author

    The role of Nod signal structures in the determination of host specificity in the Rhizobium-legume symbiosis.

    World journal of microbiology & biotechnology·2014
    Same author

    Role of plant defence in alfalfa during symbiosis.

    World journal of microbiology & biotechnology·2014
    Same author

    Interspecies homology of nodulation genes in Rhizobium.

    Plant molecular biology·2013
    Same author

    Use of a promoter-specific probe to identify two loci from the Rhizobium meliloti nodulation regulon.

    Plant molecular biology·2013

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Rhizobium meliloti strain 41 is known to possess symbiotic megaplasmids.
    • The existence and characteristics of a second megaplasmid, pRme41c, were investigated.

    Purpose of the Study:

    • To demonstrate the existence of a second megaplasmid, pRme41c, in R. meliloti strain 41.
    • To examine the transfer ability and compatibility of megaplasmids pRme41b and pRme41c.
    • To locate genes involved in surface exclusion on megaplasmid pRme41b.

    Main Methods:

    • Transposon (Tn5) tagging of megaplasmids pRme41b and pRme41c.
    • Bacterial conjugation experiments with various R. meliloti mutants and Agrobacterium tumefaciens recipients.
    • Analysis of plasmid deletions in Nod- and Fix- mutants.

    Main Results:

    • A second megaplasmid, pRme41c, with similar molecular weight to pRme41b was identified.
    • Both pRme41b and pRme41c exhibited transmissibility to R. meliloti and A. tumefaciens recipients.
    • Entry exclusion mechanisms likely prevent megaplasmid transfer into wild-type R. meliloti.
    • A DNA region on pRme41b, approximately 50 kb from nod genes, was implicated in surface exclusion and showed homology to pRme41c.
    • Symbiotic genes on pRme41c were not identified.

    Conclusions:

    • R. meliloti strain 41 possesses at least two transferable megaplasmids, pRme41b and pRme41c.
    • Megaplasmid transfer is regulated by surface exclusion mechanisms.
    • A distinct DNA region on pRme41b likely controls surface exclusion and shares homology with pRme41c.

    Related Experiment Videos