Related Experiment Video
Updated: Feb 9, 2026
09:31
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
Published on: March 22, 2016
18.4K
Draft Genome Sequence of Rhodococcus opacus Strain 04-OD7, Which Can Mobilize Phosphate
Bang-Xiao Zheng1,2,3,4, Huang-Kai Zhang5, Kai Ding6
1Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, People's Republic of China.
Genome Announcements
|June 9, 2018
Summary
This study reveals Rhodococcus opacus strain 04-OD7 can solubilize inorganic phosphate, a novel trait for the Rhodococcus genus. The genome analysis identified key phosphorus mobilization genes, supporting this capability.
Area of Science:
- Microbiology
- Genomics
- Biochemistry
Background:
- The genus Rhodococcus comprises Gram-positive bacteria with diverse metabolic capabilities.
- Inorganic phosphate solubilization is a crucial process for nutrient cycling and plant growth.
Purpose of the Study:
- To describe the draft genome of Rhodococcus opacus strain 04-OD7.
- To identify genes associated with inorganic phosphate solubilization in this strain.
- To establish inorganic phosphate solubilization as a characteristic of the Rhodococcus genus.
Main Methods:
- Whole-genome sequencing of Rhodococcus opacus strain 04-OD7.
- Bioinformatic analysis to identify genes involved in phosphorus metabolism.
- Confirmation of inorganic phosphate solubilization capacity.
Main Results:
- The draft genome sequence of Rhodococcus opacus strain 04-OD7 was successfully obtained.
- Multiple genes potentially involved in phosphorus (P) mobilization were identified within the genome.
- Rhodococcus opacus strain 04-OD7 demonstrated inorganic phosphate solubilization capacity.
Conclusions:
- Rhodococcus opacus strain 04-OD7 possesses the genetic machinery for inorganic phosphate solubilization.
- This finding expands the known functional repertoire of the Rhodococcus genus.
- The identified genes provide targets for further investigation into microbial phosphate acquisition.
Related Concept Videos
02:02Genomics
40.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.8K
01:22Phosphate Buffer
5.3K
The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
5.3K
02:30Genomic Imprinting and Inheritance
37.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
03:21Genome Size and the Evolution of New Genes
9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K
02:02Cis-regulatory Sequences
11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
16.5K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.5K