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Updated: Feb 9, 2026
08:28
High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
Published on: September 13, 2017
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Dapsone protects brain microvascular integrity from high-fat diet induced LDL oxidation
Rui Zhan1,2, Mingming Zhao2, Ting Zhou1
1Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
Cell Death & Disease
|June 9, 2018
Summary
Dapsone (DDS) protects brain microvessels from lipid oxidation damage by preventing the degradation of tight junction proteins. This drug shows promise in treating brain microvascular diseases linked to metabolic disorders.
Area of Science:
- Neuroscience
- Vascular Biology
- Pharmacology
Background:
- Atherosclerosis and abnormal lipid metabolism can lead to blood-brain barrier (BBB) leakage, contributing to stroke.
- Dapsone (DDS), an anti-inflammatory and antioxidant drug, has demonstrated vascular protective effects, but its role in protecting brain microvessels during lipid oxidation was unclear.
Purpose of the Study:
- To investigate the protective effects of Dapsone (DDS) on brain microvessels under conditions of lipid oxidation.
- To elucidate the underlying mechanisms of DDS's protective action on cerebral microvasculature.
Main Methods:
- Utilized a high-fat diet (HFD) mouse model to induce lipid oxidation and assess brain microvascular integrity.
- Conducted in vivo and in vitro experiments to evaluate the impact of DDS on microvascular leakage, LDL oxidation, and tight junction proteins.
Main Results:
- DDS significantly reduced brain microvascular leakage in HFD mice by decreasing serum oxidized low-density lipoprotein (oxLDL).
- DDS inhibited LDL oxidation in vitro and protected key tight junction proteins (ZO-1, occludin, claudin-5) in microvascular endothelial cells.
- DDS prevented abnormal degradation of ZO-1 by autophagy and reduced lysosome accumulation in vitro.
Conclusions:
- Dapsone (DDS) demonstrates a significant protective role in cerebral microvessels against lipid metabolic disorders.
- DDS shields brain microvasculature by preserving tight junction integrity, offering a novel therapeutic strategy for related diseases.
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