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Urinary cyclic AMP in spot urine of healthy children
Insights
This study establishes reference values for urinary cyclic adenosine monophosphate (cAMP) in children using spot urine samples. These findings simplify testing and provide crucial diagnostic benchmarks for pediatric health.
Area of Science:
- Pediatric Endocrinology
- Clinical Chemistry
- Biomarker Analysis
Background:
- Accurate reference values for urinary cyclic adenosine monophosphate (cAMP) are essential for pediatric health assessments.
- Traditional 24-hour urine collections are often impractical for children.
Purpose of the Study:
- To establish reference values for cAMP excretion in spot urine samples from healthy children.
- To evaluate the feasibility of using spot urine samples for cAMP measurement in pediatric diagnostics.
Main Methods:
- Collected spot urine samples between 09:00-12:00 from 143 healthy children (2-200 months).
- Corrected cAMP excretion for creatinine (UcAMP/crea)U and applied logarithmic transformation for data analysis.
- Assessed correlations between cAMP excretion, age, and body surface area.
Main Results:
- The mean (UcAMP/crea)U was 748 mumol/mol (95% CI: 254-2206 mumol/mol).
- No significant sex-based differences were observed.
- Decreasing cAMP values correlated significantly with increasing age and body surface area (r = -0.55 and r = -0.57, p < 0.001).
Conclusions:
- Spot urine collection is a practical and reliable alternative to 24-hour collections for measuring urinary cAMP in children.
- Established reference values provide valuable diagnostic standards for pediatric urinary cAMP levels.
Abstract:
We present reference values for the excretion of cAMP in spot urine collected between 09.00 and 12.00 hours in 143 healthy children aged 2-200 months. The excretion of cAMP was creatinine-corrected and expressed as a substance concentration ratio (UcAMP/crea)U due to a positive significant correlation between the excretion of cAMP and creatinine (r = 0.68, p less than 0.001). The mean value (95% significance limits) for (UcAMP/crea)U was 748 mumol/mol (254-2206 mumol/mol). A logarithmic transformation of the ratio was used, since preliminary analysis showed uneven distribution; when the logarithmic transformation was used, the data appeared evenly distributed. There was no significant difference between the results for girls and boys. The value of lg(UcAMP/crea)U was related to the age or body surface area with decreasing values at higher age or body surface area. (r = -0.55 and r = -0.57, p less than 0.001). Spot urine for measurement of urinary cAMP instead of a 24 h collection appears preferable due to the practicability of the test in children.