Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antigen Presenting Cells01:22

Antigen Presenting Cells

3.3K
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
3.3K
Allergic Reactions02:06

Allergic Reactions

32.4K
Overview
32.4K
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

1.9K
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
1.9K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
Specific Heat01:16

Specific Heat

67.6K
The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
67.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soy biodiesel emissions have reduced inflammatory effects compared to diesel emissions in healthy and allergic mice.

Inhalation toxicology·2015
Same author

Assessing the allergenic potential of molds found in water-damaged homes in a mouse model.

Inhalation toxicology·2014
Same author

Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model.

Journal of toxicology·2011
Same author

The relative allergenicity of Stachybotrys chartarum compared to house dust mite extracts in a mouse model.

Inhalation toxicology·2010
Same author

Biomarkers of acute respiratory allergen exposure: screening for sensitization potential.

Toxicology and applied pharmacology·2010
Same author

Maternal respiratory sensitization and gestational allergen exposure does not affect subsequent pup responses to homologous or heterologous allergen.

Journal of immunotoxicology·2009
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 9, 2026

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
17:35

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

40.5K

Evaluating Antigen-Specific IgE Using the Rat Basophil Leukemia Cell (RBL) Assay.

Marsha D W Ward1, Lisa B Copeland2

  • 1Cardiopulmonary-Immunotoxicology Branch, National Health and Environmental Effects Laboratory, Office of Research and Development, United States Environmental Protection Agency, Research Triangle Park, NC, USA. mwilburnw@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2018
PubMed
Summary
This summary is machine-generated.

A new rat basophil leukemia cell assay (RBL assay) helps identify unknown allergens and compare allergen potency in complex mixtures. This method offers a common platform for allergy research.

Keywords:
Allergic asthmaCross-reactivityMouse modelRBL assayRelative allergenicity

More Related Videos

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

34.1K
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
07:31

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Published on: November 1, 2014

13.0K

Related Experiment Videos

Last Updated: Feb 9, 2026

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
17:35

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

40.5K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

34.1K
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
07:31

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Published on: November 1, 2014

13.0K

Area of Science:

  • Immunology
  • Allergy Research
  • Toxicology

Background:

  • Allergic diseases, including asthma and rhinitis, affect a significant portion of the population, often triggered by inhaled allergens.
  • Current methods for assessing allergenicity in complex mixtures or for unknown substances are time-consuming and require purified antigens.
  • A need exists for a generic platform to evaluate allergenicity and compare the potency of various allergen sources.

Purpose of the Study:

  • To develop and validate a generic method for assessing the allergenicity of protein extracts and mixtures.
  • To provide a common platform for comparing the relative potency of different allergen sources.
  • To aid in the identification of unknown allergens and assess allergy induction risks from inhalation exposure.

Main Methods:

  • Development of a rat basophil leukemia cell assay (RBL assay) as a generic platform.
  • Utilizing the RBL assay to evaluate allergenicity of complex extracts and mixtures.
  • Comparing the relative potency of various allergen sources using the RBL assay.

Main Results:

  • The RBL assay provides insights into the allergenicity of complex extracts and mixtures.
  • The assay serves as a common platform for comparing the relative potency of different allergen sources.
  • This method facilitates the assessment of unknown or uncharacterized allergens.

Conclusions:

  • The rat basophil leukemia cell assay (RBL assay) is an effective generic method for assessing allergenicity.
  • This assay enables the comparison of relative allergen potency across diverse sources.
  • The RBL assay offers a valuable tool for allergy research, particularly with complex or unknown allergens.