Magnesium uptake characteristics in Arabidopsis revealed by ²⁸Mg tracer studies

The Mg²⁺ uptake system in Arabidopsis roots is Gd³⁺- and Fe²⁺-sensitive, and responds to a changing Mg²⁺ concentration within 1 h with the participation of AtMRS2 transporters. Magnesium (Mg²⁺) absorption and the mechanism regulating its activity have not been clarified yet. To address these issues, it is necessary to reveal the characteristics of Mg²⁺ uptake in roots. Therefore, we first investigated the Mg²⁺ uptake characteristics in roots of 1-week-old Arabidopsis plants using ²⁸Mg. The Mg²⁺ uptake system in roots was up-regulated within 1 h in response to the low Mg²⁺ condition. This induction was inhibited in Arabidopsis "mitochondrial RNA splicing 2/magnesium transport" mutants atmrs2-4/atmgt6 and atmrs2-7/atmgt7, while the expression of AtMRS2-4/AtMGT6 and AtMRS2-7/AtMGT7 genes in the Arabidopsis wild-type was not responsive to Mg²⁺ conditions. In addition, the Mg deficiency-induced Mg²⁺ uptake system was shut-down within 5 min when Mg²⁺ was resupplied to the environment. An inhibition study showed that the constitutive mechanism functioning in Mg²⁺ uptake under Mg²⁺ sufficient conditions was sensitive to a number of divalent and trivalent cations, particularly Gd³⁺ and Fe²⁺, but not to K⁺.