Related Experiment Video
Updated: Feb 9, 2026
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
Pathway based therapeutic targets identification and development of an interactive database CampyNIBase of
Mohammad Uzzal Hossain1, Taimur Md Omar2, Iftekhar Alam3
1Bioinformatics Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
Abstract:
The bacterial species Campylobacter jejuni RM1221 (CjR) is the primary cause of campylobacteriosis which poses a global threat for human health. Over the years the efficacy of antibiotic treatment is becoming more fruitless due to the development of multiple drug resistant strains. Therefore, identification of new drug targets is a valuable tool for the development of new treatments for affected patients and can be obtained by targeting essential protein(s) of CjR. We conducted this in silico study in order to identify therapeutic targets by subtractive CjR proteome analysis. The most important proteins of the CjR proteome, which includes chokepoint enzymes, plasmid, virulence and antibiotic resistant proteins were annotated and subjected to subtractive analyses to filter out the CjR essential proteins from duplicate or human homologous proteins. Through the subtractive and characterization analysis we have identified 38 eligible therapeutic targets including 1 potential vaccine target. Also, 12 potential targets were found in interactive network, 5 targets to be dealt with FDA approved drugs and one pathway as potential pathway based drug target. In addition, a comprehensive database 'CampyNIBase' has also been developed. Besides the results of this study, the database is enriched with other information such as 3D models of the identified targets, experimental structures and Expressed Sequence Tag (EST) sequences. This study, including the database might be exploited for future research and the identification of effective therapeutics against campylobacteriosis. URL: (http://nib.portal.gov.bd/site/page/4516e965-8935-4129-8c3f-df95e754c562#Banner).
Insights
This study identified 38 essential therapeutic targets, including one vaccine candidate, for Campylobacter jejuni RM1221 to combat antibiotic resistance and campylobacteriosis. A new database, CampyNIBase, aids future drug discovery efforts.
Area of Science:
- Microbiology
- Computational Biology
- Drug Discovery
Background:
- Campylobacter jejuni RM1221 causes global health issues (campylobacteriosis).
- Increasing antibiotic resistance necessitates novel therapeutic strategies.
- Targeting essential proteins of C. jejuni is crucial for new drug development.
Purpose of the Study:
- To identify essential therapeutic targets in C. jejuni RM1221 using in silico subtractive proteome analysis.
- To discover potential drug targets, including vaccine candidates and targets for existing drugs.
- To develop a comprehensive database (CampyNIBase) for C. jejuni research.
Main Methods:
- Subtractive proteome analysis of C. jejuni RM1221.
- Annotation and characterization of C. jejuni proteins (chokepoint enzymes, plasmids, virulence, antibiotic resistance).
- Filtering essential proteins by removing duplicates and human homologs.
Main Results:
- Identified 38 eligible therapeutic targets, including 1 potential vaccine target.
- Discovered 12 targets in interactive networks, 5 targets actionable with FDA-approved drugs, and 1 pathway-based target.
- Developed CampyNIBase, a database with target 3D models, experimental structures, and EST sequences.
Conclusions:
- The identified targets and CampyNIBase offer valuable resources for developing new campylobacteriosis therapeutics.
- This study provides a foundation for future research into effective treatments against drug-resistant C. jejuni strains.
- The findings support the development of novel anti-infective strategies targeting essential bacterial proteins.
More Related Videos
12:34Author Spotlight: Exploring ShiDuGao's Multi-Target Approach in Anus Eczema Treatment
Published on: January 12, 2024
07:38Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
Published on: April 11, 2019
Related Concept Videos
01:19Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
02:26Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
01:27C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
01:25cAMP-dependent Protein Kinase Pathways
02:57piRNA - Piwi-interacting RNAs
01:18Cardiovascular Drugs: Classification based on Therapeutic Indications