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Updated: Feb 9, 2026
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Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
Published on: June 16, 2018
6.0K
Microtopographies control the development of basal protrusions in epithelial sheets
Sylvie Coscoy1, Sarah Baiz2, Jean Octon1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR 168, 75005 Paris, France.
Biointerphases
|June 10, 2018
Summary
Epithelial cells form deep basal protrusions on micro-patterned scaffolds, especially those smaller than cell size. This controlled cell behavior advances biomaterial development and tissue engineering applications.
Area of Science:
- Cell biology
- Biomaterials science
- Tissue engineering
Background:
- Cells form membrane protrusions influencing adhesion and migration.
- Formation of basal protrusions in epithelial sheets on microtopography is not well understood.
Purpose of the Study:
- To investigate microtopography-induced epithelial cell basal protrusion formation.
- To analyze the impact of scaffold geometry and size on protrusion dynamics.
Main Methods:
- Fabrication of hexagonal lattices (1.5-19 μm size, 5-10 μm height) using two-photon photopolymerization.
- Culturing epithelial cells on these microstructured scaffolds to observe protrusion formation.
- Characterization of protrusion morphology, kinetics, and epithelial layer integrity.
Main Results:
- Cells generated numerous, extensive, and deep basal protrusions on scaffolds with hexagon sizes smaller than cell size (3-10 μm).
- A continuous epithelial layer was maintained above the structures.
- Protrusion formation kinetics were dependent on scaffold geometry and size.
Conclusions:
- 3D microtopography significantly influences epithelial cell basal protrusion formation.
- Controlled formation of these protrusions can enhance biomaterial adhesive properties.
- This research offers potential improvements for tissue engineering strategies.
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