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Updated: Feb 9, 2026

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
A lysosomal proton pump turns on when glucose runs out
Karlett J Parra1, Summer R Hayek2
1From the Department of Biochemistry and Molecular Biology, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131 kjparra@salud.unm.edu.
Abstract:
Vacuolar H+-ATPase (V-ATPase) is a glucose-responsive, ATP-driven proton pump that controls the acidity of cellular organelles. Increases in glucose stimulate V-ATPase assembly and activity, and glucose deprivation triggers rapid V-ATPase disassembly and inactivation in yeast. McGuire and Forgac describe the opposite phenomenon in mammalian cells, specifically that V-ATPase assembly and activation increases when glucose is lost, raising new questions about mammalian mechanisms of energy conservation.
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