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Related Concept Videos

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
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Related Experiment Video

Updated: Feb 9, 2026

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
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Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

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Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons.

Anna Boccaccio1

  • 1Institute of Biophysics, National Research Council, Genoa, Italy. anna.boccaccio@ge.ibf.cnr.it.

Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2018
PubMed
Summary
This summary is machine-generated.

Olfactory sensory neurons use cilia for smell detection. This study details using the patch clamp technique to study the ion channels responsible for olfactory transduction in these neurons.

Keywords:
CNGOlfactionOlfactory sensory neuronPatch clampTMEM16Voltage-gated currents

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Sensory Physiology

Background:

  • Olfactory sensory neurons (OSNs) are specialized bipolar cells responsible for detecting odors.
  • The olfactory cilia on OSNs house the molecular machinery for olfactory transduction.
  • Understanding the ionic conductances in OSNs is crucial for comprehending olfactory signaling.

Purpose of the Study:

  • To describe the preparation of dissociated olfactory neurons.
  • To detail the application of the patch clamp technique for studying OSNs.
  • To functionally characterize the ionic conductances in olfactory sensory neurons.

Main Methods:

  • Dissociation of olfactory sensory neurons from their native environment.
  • Utilization of the patch clamp technique for electrophysiological recordings.
  • Analysis of ion channel activity and receptor-mediated currents.

Main Results:

  • Successful preparation of viable dissociated olfactory sensory neurons.
  • Demonstration of the patch clamp technique's efficacy in studying OSN ion channels.
  • Characterization of specific ionic conductances underlying olfactory transduction.

Conclusions:

  • Dissociated olfactory neurons are suitable for patch clamp analysis.
  • The patch clamp technique provides valuable insights into olfactory transduction mechanisms.
  • This methodology facilitates the functional characterization of ion channels in olfactory sensory neurons.