Internal transport of CO₂ from the root-zone to plant shoot is pH dependent

We investigated the fate of carbon dioxide (CO₂ ) absorbed by roots or internally produced by respiration using gas exchange and stable isotopic labeling. CO₂ efflux from detached leaves supplied with bicarbonate/CO₂ solutions was followed over six cycles. CO₂ effluxes were detected when bicarbonate solution at high pH was used, corresponding to 71-85% of the expected efflux. No CO₂ efflux was detected when CO₂ solutions at low pH were used but CO₂ efflux was subsequently detected as soon as bicarbonate solutions at high pH were supplied. By sealing the leaf and petiole in a plastic bag to reduce diffusion to the atmosphere, a small CO₂ efflux signal (14-30% of the expected efflux) was detected suggesting that CO₂ in the xylem stream can readily escape to the atmosphere before reaching the leaf. When the root-zones of intact plants were exposed to CO₂ solutions, a significant efflux from leaf surface was observed (13% of the expected efflux). However, no signal was detected when roots were exposed to a high pH bicarbonate solution. Isotopic tracer experiments confirmed that CO₂ supplied to the root-zone was transported through the plant and was readily lost to the atmosphere. However, little ¹³ C moved to the shoot when roots were exposed to bicarbonate solutions at pH 8, suggesting that bicarbonate does not pass into the xylem.