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Published on: July 17, 2019
Temporal population genetic structure in the pollen pool for flowering time: A field experiment with Brassica rapa
Jennifer L Ison1,2, Arthur E Weis2,3
1Department of Biology, The College of Wooster, 1189 Beall Avenue, Wooster, Ohio 44691 USA.
Premise Of The Study:
Assortative mating by flowering time can cause temporal genetic structure in species with heritable flowering times. A strong temporal structure, when coupled with a seasonal shift in selection, may lead to adaptive temporal clines. We implemented a prospective and retrospective method to estimate the temporal genetic structure in the pollen pool of Brassica rapa.
Methods:
The prospective method uses flowering schedules to estimate the seasonal shift in the pollen donors' phenotype. By examining the offspring generation, we can get a direct estimate of temporal genetic structure, i.e., a retrospective estimate. However, this estimate is problematic because of the phenotypic correlation of the trait of interest, flowering time, between dam and sire. We developed a novel retrospective method that isolates flowering time by holding the maternal contribution constant and sampled the pollen pool in eight open-pollinated field plots throughout the flowering season.
Key Results:
We found temporal genetic structure for flowering time in seven of the eight field plots. Interestingly, the direct (retrospective) temporal structure estimate was 35% larger than the prospective estimate based on flowering schedules. Spatial clumping of pollen donors did not affect temporal structure, but structure intensified when heritability was experimentally enhanced.
Conclusions:
Temporal genetic structure, especially for flowering time, likely occurs in many plant populations and may be underestimated using a prospective method. We discuss the genome-wide consequences of temporal genetic structure and the potential for adaptive temporal clines in plant populations.
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