Modulation of polyamine biosynthesis in Arabidopsis thaliana by a drought mitigating Pseudomonas putida strain

Plant growth promoting rhizobacteria (PGPR) are a diverse group of beneficial soil bacteria that help plants in myriad ways. They are implicated in the processes of general growth and development, as well as stress mitigation. Although the physiology of plant-PGPR interaction for abiotic stress tolerance has been well reported, the underlying molecular mechanisms in this phenomenon are not clearly understood. Among the many endogenous molecules that have been reported to impart abiotic stress tolerance in plants are a group of aliphatic amines called polyamines. Here, we report the impact of a free living, drought-mitigating rhizobacterial strain, Pseudomonas putida GAP-P45 on the expression of key genes in the polyamine metabolic pathway and the accumulation of the three major polyamines, putrescine, spermidine and spermine in water-stressed Arabidopsis thaliana. We observed that, inoculation of A. thaliana with P. putida GAP-P45 with or without water-stress, caused significant fluctuations in the expression of most polyamine biosynthetic genes (ADC, AIH, CPA, SPDS, SPMS and SAMDC) and cellular polyamine levels at different days of analysis post treatments. The enhanced accumulation of free cellular putrescine and spermidine observed in this study correlated positively with the water stress tolerant phenotype of A. thaliana in response to P. putida GAP-P45 inoculation reported in our previous study (Ghosh et al., 2017). Our data point towards (a) transcriptional regulation of polyamine biosynthetic genes and (b) complex post transcriptional regulation and/or interconversion/canalization of polyamines, by P. putida GAP-P45 under normal and water-stressed conditions.