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Updated: Feb 9, 2026
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
Circular RNA expression profiles are significantly altered in mice bone marrow stromal cells after total body
Junhua Zhang1, Jing Jiang1, Rong Huang2
1Clinical Laboratory of The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Abstract:
The development of circular RNA (circRNA) microarrays has facilitated studies on the role of circRNAs in regulating gene expression through a circRNA-miRNA-mRNA network. In our study, a microarray was used to detect the expression profiles of circRNAs in mouse bone marrow stromal cell (MSCs) after total body irradiation (TBI) in an X-ray field. Forty mice were randomly distributed into two groups, the control group and the TBI group, with 20 mice in each. Using circRNA microarray data, we compared the circRNA expression profiles in the MSCs between the two groups. Randomly chosen differentially expressed circRNAs were validated as potential TBI biomarkers. Overall, 148 circRNAs were significantly upregulated while the other 133 were downregulated between the TBI group and the sham group. Among them, two upregulated circRNAs (mmu_circRNA_011235 and mmu_circRNA_016901) and three downregulated circRNAs (mmu_circRNA_008488, mmu_circRNA_000551 and mmu_circRNA_005365) exhibited trends comparable to microarray data in the validation experiments. The miRNA support vector regression method was used to predict potential target miRNAs for the circRNAs while ClueGO was used for functional analysis of predicted miRNA target genes. This is the first study to investigate the differential circRNA expression profile in TBI and the associated functions. Significantly upregulated or downregulated circRNAs may represent novel biomarkers and novel therapeutic targets for the clinical management of radiation injury with TBI.
Insights
This study identified significant changes in circular RNA (circRNA) expression in mouse bone marrow stromal cells following total body irradiation (TBI). These differentially expressed circRNAs show potential as biomarkers and therapeutic targets for radiation injury.
Area of Science:
- Molecular Biology
- Genomics
- Radiation Biology
Background:
- Circular RNAs (circRNAs) play roles in gene regulation via circRNA-miRNA-mRNA networks.
- Understanding circRNA expression changes after radiation exposure is crucial for managing radiation injury.
Purpose of the Study:
- To investigate differential circRNA expression profiles in mouse bone marrow stromal cells (MSCs) after total body irradiation (TBI).
- To identify potential circRNA biomarkers and therapeutic targets for TBI-induced radiation injury.
Main Methods:
- circRNA microarray analysis was performed on MSCs from control and TBI-exposed mice.
- Differentially expressed circRNAs were identified and validated.
- Bioinformatic tools (miRNA support vector regression, ClueGO) were used for target prediction and functional analysis.
Main Results:
- A total of 148 circRNAs were significantly upregulated and 133 downregulated in TBI-exposed MSCs compared to controls.
- Specific circRNAs (e.g., mmu_circRNA_011235, mmu_circRNA_016901, mmu_circRNA_008488) showed validated differential expression.
- Functional analysis predicted potential roles for these circRNAs in response to TBI.
Conclusions:
- This study provides the first comprehensive analysis of circRNA expression profiles in TBI.
- Differentially expressed circRNAs represent promising novel biomarkers and therapeutic targets for clinical management of radiation injury.
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