Related Experiment Video
Updated: Feb 9, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Published on: November 5, 2018
Noninvasive Structural Analysis of Intermediate Species During Fibrillation: An Application of Small-Angle X-Ray
Annette Eva Langkilde1, Fátima Herranz-Trillo1,2, Pau Bernadó2
1Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
Abstract:
Structural investigation of intermediately formed oligomers and pre-fibrillar species is of tremendous importance in order to elucidate the structural principles of fibrillation, and because intermediate species have been suggested as the pathogenic agents in several amyloid diseases. Structural investigations are however greatly complicated by the dynamic changes between structural states of very different sizes and life-times. Small angle X-ray scattering (SAXS) is an ideal method to handle this challenge. The method provides information about the fibrillation process (number of species present and their volume fractions) and low-resolution 3-dimensional structural models of individual species, notably also of the intermediately formed, in-process species from undisturbed fibrillation equilibria. Here, we provide a detailed description of the methods used for the measurement and analysis of SAXS data from fibrillating samples, exemplified using data from our own research.
More Related Videos
09:15Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
10:27Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
Published on: October 21, 2018
Related Concept Videos
The Structure of Intermediate Filaments
Intermediate...
Amyloid Fibrils
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
Amyloid Fibrils
X-ray Crystallography
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Structural Protein Function
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Keystone Species