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Related Experiment Videos

Platelet-derived growth factor promotes human peripheral monocyte activation.

D Y Tzeng, T F Deuel, J S Huang

    Blood
    |July 1, 1985
    PubMed
    Summary

    Platelet-derived growth factor (PDGF) activates human monocytes similarly to polymorphonuclear leukocytes (PMNs). PDGF induces superoxide anion generation, enzyme release, and cell adherence, demonstrating its role in monocyte activation.

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    Physical review letters·2020

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Platelet-derived growth factor (PDGF) is a potent mitogen and chemoattractant.
    • Polymorphonuclear leukocytes (PMNs) are key immune cells involved in inflammatory responses.
    • Monocytes play crucial roles in innate and adaptive immunity, differentiating into macrophages and dendritic cells.

    Purpose of the Study:

    • To investigate the effects of purified platelet-derived growth factor (PDGF) on human monocyte activation.
    • To compare the activation responses of monocytes induced by PDGF with those induced by formyl-methionyl-leucyl-phenylalanine (fMLP) and with responses in PMNs.

    Main Methods:

    • Purification of PDGF to homogeneity.
    • Assay of superoxide anion (O-.2) generation using a membrane-associated oxidase system.

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  • Measurement of granule enzyme release (lysozyme, beta-glucuronidase).
  • Evaluation of cell adherence and cell aggregation.
  • Dose-response studies with varying PDGF concentrations.

    • Main Results:

    • PDGF induced superoxide anion generation, lysozyme release, and enhanced cell adherence and aggregation in monocytes.
    • Superoxide anion release was maximized at 10 ng/mL PDGF, comparable to fMLP.
    • Lysozyme release and monocyte adherence increased dose-dependently, with maximal responses at 40 ng/mL PDGF.
    • Monocyte aggregation required a higher PDGF concentration (60 ng/mL) compared to PMNs.
    • A positive correlation was observed between PDGF concentration and beta-glucuronidase release in monocytes.

    Conclusions:

    • PDGF induces a comprehensive set of activation events in human monocytes.
    • The activation profile of monocytes by PDGF closely resembles that observed in PMNs.
    • PDGF is a significant factor in modulating monocyte inflammatory and immune functions.