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An Overview of the Loading Efficacy of Cationic Lipids with Milk Proteins: A Potential Application for Lipid Delivery

Penprapa Chanphai1, Philippe Bourassa1, Heidar Ali Tajmir-Riahi1

  • 1Department de Chemistry-Biochemistry, University of Quebec a Trois-Rivieres, C.P. 500, Trois-Rivieres (Quebec) G9A 5H7, Canada.

Current Medicinal Chemistry
|June 12, 2018
PubMed

In this review, the loading efficacies of helper and Cationic Lipids Cholesterol (CHOL), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), Dioctadecyl Dimethyl- Ammonium Bromide (DDAB) and Dioleoyl Phosphatidylethanolamine (DOPE) with milk β- lactoglobulin, α-casein and β-casein were compared in aqueous solution at physiological conditions. Structural analysis showed that lipids bind milk proteins via hydrophilic, hydrophobic and H-bonding contacts with DOTAP and DDAB forming more stable protein conjugates. Loading efficacy was 30-50% and enhanced with cationic lipids. Lipid conjugation altered protein conformation, causing a partial protein structural destabilization. Milk proteins are capable of transporting lipids in vitro.

Keywords:
Cationic Lipids CholesterolMilk proteinlipidloading efficacymodelingspectroscopy

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