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Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolates, Isolated from a Burn Hospital in

Ehsanollah Ghaznavi-Rad1,2, Alireza Ekrami3

  • 1Molecular Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.

International Journal of Microbiology
|June 12, 2018
PubMed
Abstract

Insights

Methicillin-resistant Staphylococcus aureus (MRSA) is a growing concern in burn patients. Molecular typing revealed ST-239 as a predominant clone, highlighting the need for improved infection control in hospitals.

Area of Science:

  • Medical Microbiology
  • Epidemiology
  • Infectious Diseases

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) incidence is rising, particularly in burn patients, leading to significant morbidity and mortality.
  • Molecular and epidemiological studies are crucial for understanding isolate relatedness in clinical and hospital settings.

Purpose of the Study:

  • To determine the molecular characteristics of MRSA isolates from burn patients in 2006 and 2014.
  • To compare MRSA strains using staphylococcal protein A (Spa) typing and Multilocus Sequence Typing (MLST).

Main Methods:

  • Analysis of 71 MRSA isolates collected from burn patients in 2006 and 2014.
  • Utilized MLST and Spa typing for molecular characterization of the isolates.

Main Results:

  • Identified Spa type t021 (ST-30-IV MRSA, PVL-positive) in 14.6% and t12366 (ST-8-IV MRSA, PVL-negative) in 9.8% of cases.
  • Spa typing in 2014 revealed five distinct spa types among MRSA isolates.
  • The predominant clones across both studies were t037, ST-239, SCCmec III, and CC8, accounting for 63% of isolates.

Conclusions:

  • The sustained predominance of ST-239 MRSA is a significant public health concern, impacting staphylococcal infection management.
  • The rapid dissemination of SCCmec type IV strains necessitates enhanced hospital infection-control policies.

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