Related Experiment Video

Updated: Feb 9, 2026

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
08:37

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Published on: November 18, 2011

27.7K

CD34 stromal expression is inversely proportional to smooth muscle actin expression and extent of morphea

J S Lee1,2,3, H S Park1,2,3, H S Yoon1,2,3

  • 1Department of Dermatology, Seoul Metropolitan Government - Seoul National University Boramae Medical Center, Seoul, Korea.

Journal of the European Academy of Dermatology and Venereology : JEADV
|June 12, 2018
PubMed
Abstract

Insights

In morphea (localized scleroderma), dermal dendritic cells (DDCs) may transform into myofibroblasts, contributing to fibrosis. Loss of CD34+ DDCs can predict disease severity.

Area of Science:

  • Dermatology
  • Immunohistochemistry
  • Pathophysiology

Background:

  • Fibrosis is a key feature of scleroderma, driven by myofibroblasts.
  • Altered dermal dendritic cells (DDCs) and vascular changes are implicated in scleroderma pathogenesis.

Purpose of the Study:

  • To explore the fibrotic pathogenesis of morphea (localized scleroderma).
  • To assess the relative expression and distribution of DDCs and myofibroblasts in morphea skin lesions.

Main Methods:

  • Immunohistochemical staining was performed on skin lesions from 50 morphea patients and 50 controls.
  • Antibodies used included CD34, factor XIIIa (FXIIIa), smooth muscle actin (SMA), CD31, and vascular cell adhesion molecule-1 (VCAM-1).

Main Results:

  • CD34 stromal staining was significantly lower in morphea patients compared to controls.
  • FXIIIa, SMA, and VCAM-1 staining were significantly higher in patients.
  • CD34 stromal staining showed a decreasing trend with increasing disease extent and fibrosis, inversely correlating with SMA staining.

Conclusions:

  • The findings suggest a phenotypic shift of CD34+ DDCs to SMA+ myofibroblasts in morphea, correlating with disease progression.
  • Reduced CD34+ DDC expression may serve as a predictive marker for morphea extent and severity.

More Related Videos

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line12:58

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Published on: May 10, 2017

9.9K
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K

Related Concept Videos

Muscles for Facial Expressions01:14

Muscles for Facial Expressions

The craniofacial muscles are a collection of approximately 20 thin skeletal muscles situated beneath the skin of the face and scalp. These muscles, primarily responsible for the vast array of human facial expressions, originate from the bones or fibrous structures of the skull and extend outwards to connect with the skin. While most skeletal muscles in the body are enveloped in thick fascia, facial muscles generally have a more delicate fascial covering, with the buccinator muscle being a...
4.9K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
Functions of Smooth Muscles01:23

Functions of Smooth Muscles

Smooth muscles are an important type of muscle tissue that plays a vital role in the involuntary movements of internal organs. For example, they help regulate the movement of food through the gut and the flow of blood through the circulatory system.
Function of visceral smooth muscles
Visceral smooth muscle is found in the walls of all hollow organs, except the heart, and is a key player in the involuntary movements that drive the functioning of these internal organs. This tissue is arranged in...
3.4K
Smooth Muscle Contraction01:25

Smooth Muscle Contraction

Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
7.9K
Sample Proportion and Population Proportion01:20

Sample Proportion and Population Proportion

Collecting samples or responses from an entire population takes significant time and effort, so a researcher collects responses from only a sample of that population. Suppose a study needs to collect information about a specific mobile application. After sample collection, the researcher analyzes the data and discovers that most individuals in the sample use that specific mobile application. The sample proportion measures the number of individuals in a sample who either use or don't use the...
6.9K
Structure and Organization of Smooth Muscles01:13

Structure and Organization of Smooth Muscles

Smooth muscle tissue is a type of muscle tissue that can be found lining various vital organs in the human body, including the lungs, blood vessels, digestive tract, and respiratory tract. This type of tissue is responsible for regulating the movements of these organs, playing crucial roles in the functioning of various systems, including the vascular, digestive, respiratory, and urinary systems.
Structure of smooth muscle cell
Smooth muscle cells are spindle-shaped with tapering ends and a...
8.8K