Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomics02:02

Genomics

40.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.8K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

37.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

16.5K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.5K
Sequences01:29

Sequences

281
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
281

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially resolved transcriptome-metabolome integration reveals region-specific glial lipid dysregulation associated with Alzheimer's pathology.

bioRxiv : the preprint server for biology·2026
Same author

PURE-seq integrates FACS and PIP-seq for single-cell genomics of ultra-rare cells.

Nature communications·2026
Same author

Deterministic cell pairing with simultaneous microfluidic merging and sorting of droplets.

Lab on a chip·2025
Same author

Tumor heterogeneity underlies clinical outcome and MEK inhibitor response in somatic NF1-mutant glioblastoma.

JCI insight·2025
Same author

Myeloid-mesenchymal crosstalk drives ARG1-dependent profibrotic metabolism via ornithine in lung fibrosis.

The Journal of clinical investigation·2025
Same author

Comprehensive Cross-Domain Taxonomic Classification of Microbiotas using Partitioned Amplification Multiplexed Amplicon Sequencing (PAMA-seq).

bioRxiv : the preprint server for biology·2025
Same journal

Reliability of A Vibration-Based Elastography Protocol For Assessing Achilles Tendon Stiffness Across Multiple Joint Angles In Elite Athletes.

Journal of visualized experiments : JoVE·2026
Same journal

Associations of Inflammatory and Coagulation Biomarkers with Kidney Injury Across Chronic and Acute Clinical Settings.

Journal of visualized experiments : JoVE·2026
Same journal

Intelligent Recommender Systems for Chinese Super League Fan Consumption Behavior Prediction.

Journal of visualized experiments : JoVE·2026
Same journal

A Battery of Quantitative Binocular Vision Tests for Adults: Testing Protocols.

Journal of visualized experiments : JoVE·2026
Same journal

Efficacy Analysis of Paiteling in Treating Persistent High-Risk Human Papillomavirus after Cervical Cancer Surgery.

Journal of visualized experiments : JoVE·2026
Same journal

Clinical Efficacy of Tissue-Bone Homeostasis Manipulation on Soft Tissue Balance and Function in Knee Osteoarthritis.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Feb 9, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.3K

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing.

Benjamin Demaree1, Daniel Weisgerber2, Freeman Lan1

  • 1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco; UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco.

Journal of Visualized Experiments : Jove
|June 12, 2018
PubMed
Summary
This summary is machine-generated.

We developed single-cell genome sequencing (SiC-seq) using droplet microfluidics to overcome limitations in analyzing cellular heterogeneity. This high-throughput method enables sequencing over 50,000 single cells per run for broader genomic studies.

More Related Videos

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

12.1K
Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

671

Related Experiment Videos

Last Updated: Feb 9, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.3K
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

12.1K
Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

671

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Cellular heterogeneity is crucial in biological systems, driving a shift towards single-cell resolution in sequencing.
  • Analyzing large cell populations at the single-cell level has been challenging due to genome processing limitations.

Purpose of the Study:

  • To introduce a novel method, single-cell genome sequencing (SiC-seq), for high-throughput analysis of individual cell genomes.
  • To address the limitations in processing large numbers of cells for single-cell genome sequencing.

Main Methods:

  • Utilizes droplet microfluidics for isolating, amplifying, and barcoding single-cell genomes.
  • Employs microgels for compartmentalized DNA purification and tagmentation.
  • Incorporates a microfluidic merger to assign unique oligonucleotide barcodes to each genome.

Main Results:

  • Enables sequencing of over 50,000 single cells per run.
  • Generates demultiplexed sequencing data, with reads grouped by single-cell origin.
  • Provides a high-throughput and low-bias method for single-cell genome sequencing.

Conclusions:

  • SiC-seq significantly advances the capability to study cellular heterogeneity.
  • The method facilitates a wider range of genomic studies across diverse cell populations.
  • SiC-seq is poised to become a valuable tool in molecular biology and genomics research.